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The goal of the Gene Expression and Bioinformatics Core will
be to provide expression arrays of muscle mRNAs to examine
differential mRNA expression in various developmental states
of unaffected individuals, muscle disease states and differences
between stem cell populations at different stages of differentiation.
The Core will also provide web-accessible annotation, cataloging
facilities and state-of-the-art bioinformatics analyses. This
will enable researchers from all Projects to maximally utilize
the expression data sets to determine functional dependencies
among the known genes and Expressed Sequence Tags (ESTs) and
direct further biological validation of these putative dependencies.
This critical biological validation step is described in each
of the respective Project proposals. The facility will use
two complementary microarray methodologies. The first methodology
will be Affymetrix GeneChip arrays of human expressed sequences.
The second will be the gridded array of normalized human muscle
cDNA clones on microscope slides.
The Aims of this Core are to:
- establish the web-based data portal
- standardize gene expression analysis technology
- develop benchmark data sets for gene expression in normal
human muscle
- use bioinformatic techniques to identify functional
dependencies between interacting proteins.
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